Plasmid_Backbone

Part:BBa_K514004:Design

Designed by: Elena Moreno Martínez   Group: iGEM11_Sevilla   (2011-09-25)


GeneArt pMK-RQ (Kan)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1994
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2308
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal PstI site found at 1994
    Illegal NotI site found at 9
    Illegal NotI site found at 2314
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2308
    Illegal BglII site found at 1626
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2308
    Illegal suffix found at 2
    Illegal PstI site found at 1994
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2308
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2323
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal PstI site found at 1994
    Illegal NgoMIV site found at 1848
    Illegal AgeI site found at 1955
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


Design Notes

None


Source

GeneArt, DNA synthesis company

References